Regulation of the iron transporter gene fepA is crucial for cell viability


Research Paper (postgraduate), 2010

9 Pages, Grade: 1,0


Abstract or Introduction

FepA is an Escherichia coli (E. coli) membrane protein which transports the iron-bound siderophore ferric enterobactin from the exterior into the periplasm. Thereby it helps to provide the cells with soluble iron. In the study, its gene, fepA, was ligated into pUC8. E. coli cells were then transformed with this construct and tested for presence and orientation of sense or antisense expression. The majority of colonies that have taken up pUC8 with a fepA fragment were found to contain the gene in antisense orientation. It was concluded that removal of the gene from its regulation leading to its over-expression results in unviable clones possibly due to membrane protein toxicity or iron toxicity.

Details

Title
Regulation of the iron transporter gene fepA is crucial for cell viability
College
Washington State University
Course
Techniques in Molecular Biology
Grade
1,0
Author
Year
2010
Pages
9
Catalog Number
V175339
ISBN (eBook)
9783640965359
ISBN (Book)
9783640965304
File size
574 KB
Language
English
Keywords
regulation
Quote paper
Eva Weiss (Author), 2010, Regulation of the iron transporter gene fepA is crucial for cell viability , Munich, GRIN Verlag, https://www.grin.com/document/175339

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