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The Influence of 3-Hydroxybutyrate and Microcurrent Treatment on Cardiomyocytes during Simulated Hypertrophy


Bachelor Thesis, 2010

31 Pages, Grade: 2,0


Excerpt


Table of Contents

Abstract

Zusammenfassung

Introduction
Theoretical Background
Chronic Heart Failure
Mitochondrial Alterations
Metabolic Shift
Insulin Resistance
Ketone Bodies
Oxidative Stress
Microcurrent Therapy
Aim of this experiment

Results
Hypertrophic Model
H9c2
Primary cardiomyocytes (SHR7)
Electro Microcurrent treated primary (SHR7) cells
Cell growth under ß-Hydroxybutyrate treatment
Cell proliferation Assay with alamarBlue®
Hypertrophic growth under ß-Hydroxybutyrate treatment
ROS assay
Immunohistochemical staining (Mitochondrial distribution)
H9c2
SHR7

Discussion
Hypertrophic Model
Mitochondrial Activity Assay
ROS Assay
Immunostaining

Conclusion

References

Eigenständigkeitserklärung

Attachment:
Abbreviations
Materials
Methods
Hypertrophic Model
Hypertrophic Measurement
HE-Staining
MitoTracker®
ROS-Measurement
OHB Proliferation Assay
AlamarBlue®-Assay
Cristal Violet-Assay
Immunostaining (Caveolin-3)
Immunostaining (a-Tubulin)
Microcurrent treatment of SHR7 cells

Excerpt out of 31 pages

Details

Title
The Influence of 3-Hydroxybutyrate and Microcurrent Treatment on Cardiomyocytes during Simulated Hypertrophy
College
FH Campus Vienna - University of Applied Sciences  (Molekulare Biotechnologie)
Grade
2,0
Author
Year
2010
Pages
31
Catalog Number
V168718
ISBN (eBook)
9783640869084
ISBN (Book)
9783640869558
File size
1244 KB
Language
English
Notes
Eine Bachelorarbeit, die den Einfluss von 3-Hydroxybutyrate und geringen Strombehandlungen auf die Entwicklung von Hypertrophie in primären Zellkulturen sowie Zelllinien untersucht. Der theoretische Teil fasst die aktuellen Erkenntnisse über die Entwicklung von Hypertrophie und chronischem Herzversagen auf zellulärer Ebene zusammen.
Keywords
Hypertrophie, Herzmuskel, chronic heart failure, 3-hydroxybutyrate, microcurrent treatment, H9c2
Quote paper
BSc Matthias Pilecky (Author), 2010, The Influence of 3-Hydroxybutyrate and Microcurrent Treatment on Cardiomyocytes during Simulated Hypertrophy, Munich, GRIN Verlag, https://www.grin.com/document/168718

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